Altering Ca2induced exocytosis and its metabolic amplification. AUTHOR’S CONTRIBUTIONS LRBS, CM, HKT, and JCJ conceived the study and created the experiments. LRBS, CM, AHS, HKT, and HC performed the experiments and analysed the information, except for OCR measurements (IRS) and GC/MS evaluation (PS and HM). LRBS, CM, and JCJ wrote the paper, other authors edited and authorized it. ACKNOWLEDGEMENTSWe thank Dr. TingeTing Huang (Stanford Neuroscience Institute, CA) for mouse NNT antibody, and Dr. Decio L. Eizirik (UniversitLibre de Bruxelles, Belgium) for the protocol of adenoviral infection immediately after gentle trypsinization. JCJ is Study Director of your Fonds de la Recherche Scientifique-FNRS, Belgium. AHS was a recipient of scholarship abroad (BEPE-DR #2013/18232-5) from S Paulo Research Foundation (FAPESP). This study was funded by Action de Recherche Concert 12/17-047 in the Communautfran ise de Belgique, Grant 3.4521.12 from the Fonds de la Recherche Scientifique M icale, and Grant SFD/MSD 2016 from the Soci Francophone du Diab e (Paris, France) to JCJ, as well as the National Institutes of Health Grant DK17047 (the DRC Cell Function Analysis Core) to IRS. An gear grant from Knut and Alice Wallenberg’s Foundation is acknowledged.CONFLICT OF INTERESTThe authors declare that they have no conflict of interest.2017 The Authors. Published by Elsevier GmbH. This can be an open access write-up below the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Original ArticleAPPENDIX A. SUPPLEMENTARY DATASupplementary information connected to this short article can be found at http://dx.doi.org/10.1016/j. molmet.2017.04.004. [19] Quoix, N., Cheng-Xue, R., Guiot, Y., Herrera, P.L., Henquin, J.C., Gilon, P., 2007. The GluCre-ROSA26EYFP mouse: a brand new model for uncomplicated identification of living pancreatic a-cells. FEBS Letters 581:4235e4240. [20] Roma, L.P., Duprez, J., Jonas, J.C., 2015. Glucokinase activation is advantageous or toxic to cultured rat pancreatic islets depending on the prevailing glucose concentration. American Journal of Physiology. Endocrinology and Metabolism 309:E632eE639. [21] Khaldi, M.Z., Guiot, Y., Gilon, P., Henquin, J.C., Jonas, J.C., 2004. Enhanced glucose sensitivity of both triggering and amplifying pathways of insulin secretion in rat islets cultured for 1 week in high glucose.(S)-(-)-tert-Butylsulfinamide structure American Journal of Physiology.Ethyl 5-bromo-1H-imidazole-2-carboxylate uses Endocrinology and Metabolism 287:E207eE217.PMID:23907051 [22] Gutscher, M., Pauleau, A.L., Marty, L., Brach, T., Wabnitz, G.H., Samstag, Y., et al., 2008. Real-time imaging of the intracellular glutathione redox potential. Nature Methods 5:553e559. [23] Sweet, I.R., Cook, D.L., DeJulio, E., Wallen, A.R., Khalil, G., Callis, J., et al., 2004. Regulation of ATP/ADP in pancreatic islets. Diabetes 53:401e409. [24] Jung, S.R., Kuok, I.T., Couron, D., Rizzo, N., Margineantu, D.H., Hockenbery, D.M., et al., 2011. Reduced cytochrome C is an important regulator of sustained insulin secretion by pancreatic islets. The Journal of Biological Chemistry 286:17422e17434. [25] Fontaine, D.A., Davis, D.B., 2016. Attention to background strain is essential for metabolic Analysis: C57BL/6 and the international knockout mouse consortium. Diabetes 65:25e33. [26] Meyer, A.J., Dick, T.P., 2010. Fluorescent protein-based redox probes. Antioxidants Redox Signaling 13:621e650. [27] Cameron, W.D., Bui, C.V., Hutchinson, A., Loppnau, P., Graslund, S., Rocheleau, J.V., 2016. Apollo-NADP(: a spectrally tunable family members of genetically encoded sensors for NADP(. Nature Me.