Te, 12.5 glycerol, 0.1 Triton X-100, protease inhibitor mixture, and 1 mM phenylmethylsulfonyl fluoride
Te, 12.five glycerol, 0.1 Triton X-100, protease inhibitor mixture, and 1 mM phenylmethylsulfonyl fluoride). Cells had been broken for 20 min with glass beads and centrifuged for ten min at…